New oligodendrocytes exhibit more abundant and accurate myelin regeneration than those that survive demyelination
Sarah A. Neely, Jill M. Williamson, Anna Klingseisen, Lida Zoupi, Jason J. Early, Anna Williams & David A. Lyons
14 February 2022
Selective vulnerability of inhibitory networks in multiple sclerosis
Lida Zoupi, Sam A. Booker, Dimitri Eigel, C. Werner, P. Kind, T. Spires-Jones, B. Newland, Anna C. Williams
15 January 2021
A rodent model of focal subpial cortical demyelination reproduces a selective vulnerability of inhibitory interneurons in multiple sclerosis, providing a new preclinical model for the study of neuroprotective treatments.
AbstractCryogel scaffolds for regionally constrained delivery of lysophosphatidylcholine to central nervous system slice cultures: a model of focal demyelination for multiple sclerosis research.
Dimitri Eigel, Lida Zoupi, S. Sekizar, P. Welzel, C. Werner, Anna C. Williams, B. Newland
1 October 2019
This manuscript is the first report of using macroporous hydrogels (cryogels) as a research tool for lysophosphatidylcholine (LPC) delivery, in order to create an ex-vivo model of focal demyelination in the brain and spinal cord, which is of great relevance to multiple sclerosis research.
AbstractAlkyne-Tagged PLGA Allows Direct Visualization of Nanoparticles In Vitro and Ex Vivo by Stimulated Raman Scattering Microscopy
Sally Vanden-Hehir, Stefan A. Cairns, Martin Lee, Lida Zoupi, M. Shaver, V. Brunton, Anna C. Williams, A. Hulme
13 August 2019
It is shown how small chemical labels can be appended to poly(lactic acid-co-glycolic acid) (PLGA) to synthesize NPs that can be imaged by stimulated Raman scattering microscopy, a vibrational imaging technique that can elucidate bond-specific information in biological environments, such as the identification of alkyne signatures in modified PLGA terpolymers.
AbstractThe Lysosomal Transcription Factor TFEB Represses Myelination Downstream of the Rag-Ragulator Complex.
A. Meireles, Kimberle Shen, Lida Zoupi, H. Iyer, Ellen L Bouchard, Anna C. Williams, W. Talbot
1 November 2018
It is shown that the lysosomal G protein RagA is essential for CNS myelination, and TFEB expression is increased in oligodendrocytes, but the protein is localized to the cytoplasm, and hence inactive, especially during remyelination.
AbstractThe function of contactin‐2/TAG‐1 in oligodendrocytes in health and demyelinating pathology
Lida Zoupi, M. Savvaki, Katerina Kalemaki, Ilias Kalafatakis, K. Sidiropoulou, D. Karagogeos
1 March 2018
A novel, CNTN2‐independent mechanism is revealed that is able to recluster voltage gated potassium channels (VGKCs) resulting in the improvement of fiber conduction during remyelination and during development, this molecule can transiently affect the expression levels of myelin and myelin‐regulating genes.
AbstractInhibitory axons are targeted in hippocampal cell culture by anti-Caspr2 autoantibodies associated with limbic encephalitis
Delphine Pinatel, B. Hivert, J. Boucraut, M. Saint-Martin, V. Rogemond, Lida Zoupi, D. Karagogeos, J. Honnorat, C. Faivre-Sarrailh
9 July 2015
Using anti-Caspr2 antibodies from seven patients affected by pure LE, it is determined that IgGs in the cerebrospinal fluid of four out seven patients were selectively directed against the N-terminal Discoïdin and LamininG1 modules of Caspr2.
AbstractAlterations of juxtaparanodal domains in two rodent models of CNS demyelination
Lida Zoupi, K. Markoullis, K. Kleopa, D. Karagogeos
1 August 2013
This study has analyzed the alterations of TAG‐1, Caspr2, and voltage‐gated potassium channels, forming the juxtaparanodal tripartite complex, in relation to adjacent paranodal and nodal molecules, in two different models of CNS demyelination, the experimental autoimmune encephalomyelitis (EAE) and the cuprizone model of toxic demyelination.
AbstractAxons and myelinating glia: An intimate contact
Lida Zoupi, M. Savvaki, D. Karagogeos
1 September 2011
Recent evidence on the key players of axo‐glial interactions is outlined, depicting their importance in myelinated fiber physiology and disease.
AbstractThe Expression of TAG-1 in Glial Cells Is Sufficient for the Formation of the Juxtaparanodal Complex and the Phenotypic Rescue of Tag-1 Homozygous Mutants in the CNS
M. Savvaki, K. Theodorakis, Lida Zoupi, A. Stamatakis, S. Tivodar, K. Kyriacou, F. Stylianopoulou, D. Karagogeos
20 October 2010
Ulastructural and behavioral analysis of tagged mice shows that the expression of glial TAG-1 is sufficient to restore the axonal and myelin deficits as well as the behavioral defects observed in Tag-1−/− animals, highlighting the pivotal role of myelinating glia on axonal domain differentiation and organization.
Abstract